Contents
Download PDF
pdf Download XML
987447 Views
42188 Downloads
Share this article
Review Article | Volume 3 Issue 1 (, 1997) | Pages 105 - 110
Chemical assay of glutaraldehyde incorporation into pericardial tissue
 ,
 ,
 ,
 ,
1
Biological & Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Under a Creative Commons license
PMID : -8162206
Published
Jan. 19, 1994
Abstract

This paper describes the development of a chemical assay to determine the incorporation of glutaraldehyde into pericardial tissue. The procedure is based upon the hydrolysis of the Schiff's bases formed between the glutaraldehyde and the E-amino groups of lysine, the liberated glutaraldehyde being determined as its 2,4-dinitrophenyl-hydrazone by high performance liquid chromatography (HPLC). The HPLC system uses a reverse phase column eluted with a gradient of aqueous acetonitrile (from 60%-70% over 30 minutes). The glutaraldehyde 2,4-dinitrophenylhydrazone is detected at 356 nm. The results show that the assay has sufficient sensitivity (minimum detectable limit 20 pmol of glutaraldehyde 2,4-dinitrophenyl-hydrazone) and reproducibility (+/- 9% at 100 pmol +/- 4% at 1 nmol) to estimate glutaraldehyde incorporation into pericardial tissue. A series of subsequent experiments have documented that temperature and buffer pH are the main determinants affecting glutaraldehyde incorporation into pericardial tissue. Immersion of pericardial tissue in glutaraldehyde solution of pH 9 and 37 degrees C yields an approximately six times greater incorporation than can be achieved if the reaction is performed at 0 degrees C. Further study is underway to relate the amount of Schiff's base bound to that of total incorporated glutaraldehyde.

 

 

 

How to cite: Hughes, H., Lilburn, S., Tipton, S., Aboul-Enein, H. Y., & Duran, C. M. (1994). Chemical assay of glutaraldehyde incorporation into pericardial tissue. The Journal of heart valve disease3(1), 105–110.

Keywords
Recommended Articles
Research Article
Human pulmonary valve endothelial cells express functional adhesion molecules for leukocytes
...
Published: 19/09/2003
Download PDF
Read Article
Research Article
Mitral Valve Regurgitation: Emerging Therapeutic Approaches
...
Published: 30/12/2018
Download PDF
Read Article
Research Article
Innovative Anticoagulation Strategies for Mechanical Heart Valves
...
Published: 30/12/2021
Download PDF
Read Article
Research Article
Three-Dimensional Printing in Heart Valve Disease: Future Perspectives
...
Published: 28/12/2020
Download PDF
Read Article
Chat on WhatsApp
© Copyright Journal of Heart Valve Disease https://icr-heart.com/