Aim of study This study examines the occurrence of EBV infection in glioma tumors that arise in individuals with a history of peripheral multiple sclerosis.the Epstein-Barr virus (EBV) has been implicated in the pathogenesis of glioma, contributing to the formation of tumors.Patients and methods we did a retrospective analysis of archival material of 30 individuals who got gliomas as a result of Multiple sclerosis. EBV DNA sequence was examined using real-time polymerase chain reaction (qPCR).   The presence of Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) was identified using quantitative polymerase chain reaction (qPCR).recorded were the clinic-pathological characteristics.A statistical analysis was conducted to compare patients who tested positive for EBV with those who tested negative for EBV.Results in total, six gliomas tested positive for the Epstein-Barr virus (EBV), which accounted for 20% of the cases.   EBV DNA was detected in 6 instances.  EBNA-LP was identified in every patient who tested positive for EBV DNA.   All patients who tested positive for Epstein-Barr virus (EBV) were diagnosed with glioblastomas multiforme (GBM).  EBV-negative patients exhibited superior median overall survival and recurrence-free survival compared to EBV-positive patients. Conclusion taken together, these results lend credence to the idea that EBV infection is present in Iraqi GBM. EBV infection also appears to be linked to a poorer prognosis for patients, at least when comparing EBV-positive and negative malignancies. The results are promising, but more advanced molecular research is needed to corroborate them and offer light on the possible function of EBV in these fatal cancers.