Aim of study multiple sclerosis (MS) is an autoimmune disorder affecting the central nervous system (CNS). This study aims to examine the CCL5 values in patients with Multiple Sclerosis. Patients and methodsA retrospective study was undertaken on 70 individuals diagnosed with multiple sclerosis utilising archived material. The RNA sequence of CCL5 was analysed using the real-time polymerase chain reaction (qPCR) technique. The CCL5 level was determined using quantitative polymerase chain reaction (RT-PCR) methodology. Results in total, a collection of 70 samples was obtained. The concentration of CCL5 was identified in all seventy patients and subsequently compared with a set of 30 control samples. There is an observed increase in the levels of CCL5 in patients diagnosed with Multiple Sclerosis (M.S) compared to individuals in good condition. Conclusion these findings provide support for the elevated levels of CCL5 observed in patients with Multiple Sclerosis. Moreover, compared to control cases, it appears that CCL5 correlates with a more unfavourable outcome in patients.